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By Donald L. Sparks

Advances in Agronomy is still well-known as a number one reference and a major resource for the most recent study in agronomy. As continuously, the themes lined are various and exemplary of the myriad of material handled through this long-running serial. * continues the top impression issue between serial courses in agriculture * offers well timed experiences on vital agronomy concerns * Enjoys a long-standing popularity for excellence within the box

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Therefore, care must be taken to develop a strong rationale for the cancer site and animal model chosen for investigation. This process should take into account the characteristics of the crop being investigated and epidemiological evidence relative to the effects of that crop on patterns of cancer occurrence in human populations. Key considerations in designing a robust experiment include (1) selecting an appropriate animal model to evaluate the hypothesis; (2) selecting an appropriate diet formulation into which a crop will be incorporated; (3) procuring materials to formulate the diets or identifying a vendor who custom formulates diets; (4) writing a detailed experimental protocol for use of animals with a well-justified design and powers calculations; (5) devising a plan for general animal care including selection of caging type, frequency of cage changing, and decisions on the frequency of data collection, for example, food intake and/or body weights; (6) duration of the experiment, methods for euthanasia, and sample collection; (7) methods for evaluation of animal tissues; and (8) plan for data analysis and interpretation.

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