Download Advances in Structural Biology, Vol. 4 by S.K. Malhotra PDF

By S.K. Malhotra

The current quantity keeps the rage tested in prior volumes during this sequence on Advances in Structural Biology. As some time past, assorted themes of present value correct to the subject matter of the sequence are integrated within the fourth quantity.

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Although he considered them to be possible "aqueous channels across the unit membrane," he did not draw any conclusion about these structures. Using a different technique of negative staining with phosphotungstic acid of isolated rat liver membranes, Benedetti and Emmelot (1965) also reported a similar hexagonal array of subunits in the membranes.

D. Molecular Components of the Tight Junction It has been hypothesized that the freeze-fracture strands seen at the tight junction are inverted cylindrical lipid micelles lying within a fusion of the outer membrane leaflets of neighboring cells (Kachar and Reese, 1982). However, tight junction 28 ANDREW G. HOWARTH and BRUCE R. STEVENSON strands are observed in negatively stained liver plasma membrane preparations after treatment with the detergent deoxycholate (Goodenough and Revel, 1970; Stevenson and Goodenough, 1984), and in hair cells of the chick ear after treatment with Triton X- 100 (Hirokawa and Tilney, 1982).

Molecular Constituents . . . . . . . . . . . . . . . A. Identification o f Gap Junction Proteins: The Connexins . . . . . . B. eDNA Analysis o f Connexins and the Connexin Multigene Family . . . C. Topological Model o f Connexin Molecules . . . . . . . . . D. Nomenclature o f Gap Junction Proteins . . . . . . . . . . E. Connexin Genes and their Chromosomal Location . . . . . . . Tissue Distribution o f Gap Junctions . . . . .

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